BioChem - DOS

Isolation and characterization of a novel hydrogen-producing strain Clostridium sp. suitable for immobilization

International journal of hydrogen energy

Nomura, T.; Naimen, A.; Toyoda, S.; Kuriyama, Y.; Tokumoto, H.; Konishi, Y.

A hydrogen-producing strain of bacteria suitable for immobilization was isolated from anaerobic sludge obtained from a methane fermentation plant. The isolated strain, CFPA-20 was identified as a novel species of the genus Clostridium by phylogenetic analysis of the 16S rRNA sequence. The changes in free energy of interaction of adhesion to polymer resin and self-aggregation were both negative. This indicated that CFPA-20 was thermodynamically favored for immobilization. CFPA-20 grew at a temperature range of 25-37 <SUP>o</SUP>C and at a pH range of 4.5-9.0. Immobilization of CFPA-20 on block copolymer polyethylene glycol-b-polypropylene glycol gave a radically improved hydrogen production yield (2.91 mol/mol-glucose) and a maximum hydrogen production rate (568 mL/L-culture/h) compared to the non-immobilized isolate. In addition, the biofilm of CFPA-20 acquired tolerance for volatile fatty acids. Further investigation into this mechanism may ultimately improve the hydrogen production capacity of CFPA-20.

2014

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

39

3

pp.1280-1287

10.1016/j.ijhydene.2013.10.166

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68286770

Hydrogen-producing strain; Anaerobic sludge; Immobilization; Free energy of interaction; Tolerability