Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae

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논문

Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Sasaki, K.; Tsuge, Y.; Sasaki, D.; Teramura, H.; Inokuma, K.; Hasunuma, T.; Ogino, C.; Kondo, A.

초록

Mechanical milling and membrane separation were applied to simultaneous saccharification and co-fermentation from hydrothermally pretreated rice straw. Mechanical milling with minimized 4 cycles enabled 37.5+/-3.4gL-1 and 45.3+/-4.4gL-1 of ethanol production after 48h by xylose-fermenting Saccharomyces cerevisiae from solid fractions (200 and 250gL-1) of pretreated rice straw with 5 filter paper unitg-biomass-1 cellulase (respectively, 77.3+/-7.1% and 74.7+/-7.3% of theoretical ethanol yield). Use of a membrane-based process including nanofiltration and ultrafiltration increased the sugar concentrations in the liquid fraction of pretreated rice straw and addition of this liquid fraction to 250gL-1 solid fraction increased ethanol production to 52.0+/-0.4gL-1 (73.8+/-0.6% of theoretical ethanol yield). Mechanical milling was effective in increasing enzymatic hydrolysis of the solid fraction and membrane separation steps increased the ethanol titer during co-fermentation, leading to a proposal for combining these processes for ethanol production from whole rice straw.

발행연도

2015

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

185

페이지

pp.263-268

DOI

10.1016/j.biortech.2015.02.117