초록
A kinetic model of fermentative hydrogen (H<SUB>2</SUB>) production was developed for the hyperthermophilic marine bacterium Thermotoga neapolitana DSMZ 4359<SUP>T</SUP> using glucose as the substrate. The model was built based on anaerobic digestion model no.1 (ADM1) by IWA using Monod-like kinetic equations. Several series of pH controlled batch fermentation tests were performed under pure CO<SUB>2</SUB> or N<SUB>2</SUB> atmosphere in serum bottles to evaluate the growth kinetic parameters. The kinetic parameters were estimated by applying the non-linear least square method to Monod and Michaelis-Menten kinetic equations. The experimentally estimated value of k, k<SUB>S</SUB>, Y and k<SUB>d</SUB> were 0.839 h<SUP>-1</SUP>, 1.42 g/L, 0.1204 and 0.0043 h<SUP>-1</SUP>, respectively. The kinetic parameters were analyzed for sensitivities and subsequently calibrated and validated by a separate set of experimental results. The model was particularly effective in estimating the biomass growth, substrate consumption, and H<SUB>2</SUB> production except for acetate and lactate production, due to capnophilic condition (under CO<SUB>2</SUB> atmosphere).