BioChem - DOS

Screening and bioprospecting of anaerobic consortia for biohydrogen and volatile fatty acid production in a vinasse based medium through dark fermentation

Process biochemistry

Sydney, Eduardo Bittencourt; Novak, Alessandra Cristine; Rosa, Drielly; Pedroni Medeiros, Adriane Bianchi; Brar, Satinder Kaur; Larroche, Christian; Soccol, Carlos Ricardo

<P><B>Abstract</B></P> <P>The use of microbial consortia and industrial effluents for the production of biohydrogen by dark fermentation is seen as a key strategy in an attempt to overcome the economic and technical drawbacks of this potential technology. Three mesophilic microbial consortia were sampled and identified. Fermentation was carried out in a vinasse-based medium supplemented with pure or complex carbon sources under different conditions of H<SUB>2</SUB> partial pressure. Consortia LPBAH1 and LPBAH2 were predominantly composed of <I>Oxalobacteraceae</I> and <I>Lactobacillaceae</I>, while LPBAH3 was rich in sporulating <I>Lactobacillaceae</I> (>96%). Each consortium presented specificities related to biohydrogen and VFAs production: (i) the highest biohydrogen yield was achieved with LPBAH1 (>50% <I>Oxalobacteraceae</I>) in a vinasse medium supplemented with sugarcane juice (1.59 &plusmn; 0.21 mol<SUB>H2</SUB>/mol<SUB>glucose</SUB>); (ii) The lower H<SUB>2</SUB> yields were achieved with LPBAH3, which otherwise produced the highest amount of butyric acid (up to 10 g L<SUP>&minus;1</SUP>); (iii) LPBAH2 presented great stability in H<SUB>2</SUB> production in different conditions of H<SUB>2</SUB> partial pressure.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three hydrogen producer mesophilic anaerobic consortia were identified. </LI> <LI> The metabolic effects of different carbon sources and H<SUB>2</SUB> partial pressure were evaluated. </LI> <LI> <I>Oxalobacteraceae</I> was present in consortia with higher biohydrogen yield. </LI> <LI> <I>Sporolactobacillaceae</I> containing consortium yielded high amount of butyric acid (10 g L<SUP>&minus;1</SUP>) but the lowest biohydrogen yields. </LI> <LI> Unprecedented yield of 1.59 mol<SUB>H2</SUB>/mol<SUB>glucose</SUB> in vinasse medium was achieved. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2018

Elsevier

1359-5113

67

pp.1-7

10.1016/j.procbio.2018.01.012

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART81670098

Anaerobic fermentation; Mixed culture; Biohydrogen; Vinasse; Volatile fatty acids