초록
<P><B>Abstract</B></P> <P>The economics of bio-solvent production are largely dependent on the cost of the fermentation substrate. <I>Dioscorea zingiberensis</I> C.H. Wright (DZW), the main raw material used to produce saponin, contains 13–18% starch which can be directly saccharified to a saccharification liquid of DZW starch (SLDS) that contains abundant nutrients. In this study, the water-soluble micromolecule compounds in SLDS were quantified through <SUP>1</SUP>H NMR. Using SLDS as the substrate to conduct ABE fermentation by <I>Clostridium beijerinckii</I>, the fermentation cycle was shortened 24h, the maximum biomass and consumption rate of the glucose significantly increased, and the productivity of total solvents were increased by 0.244±0.010g/L/h compared to standard P<SUB>2</SUB> medium. Expression analysis of genes encoding key enzymes involved in acetone and butanol production and glucose consumption showed that they were induced by SLDS. Taken together, SLDS is a useful and renewable glycosylated solution for ABE fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SLDS shortened the ABE fermentation cycle 24h. </LI> <LI> SLDS promoted <I>C. beijerinckii</I> consumption of glucose. </LI> <LI> SLDS improved the production and the productivity of acetone and butanol. </LI> <LI> The water-soluble micromolecule compounds in SLDS were quantified by <SUP>1</SUP>H NMR. </LI> <LI> SLDS induced the gene expression of <I>PTS</I>, <I>pyk</I>, <I>AADC</I> and <I>BDH</I> in <I>C. beijerinckii</I>. </LI> </UL> </P>