BioChem - DOS

Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Li, Qing; Huang, Bing; Wu, Hui; Li, Zhimin; Ye, Qin

<P><B>Abstract</B></P> <P>Glycerol is an important resource for production of value-added bioproducts due to its large availability from the biodiesel industry as a by-product. In this study, two metabolic regulation strategies were applied in the aerobic stage of a two-stage fermentation to achieve high metabolic capacities of the <I>pflB ldhA</I> double mutant <I>Escherichia coli</I> strain overexpressing phosphoenolpyruvate carboxykinase (PCK) in the subsequent anaerobic stage: use of acetate as a co-carbon source of glycerol and restriction of oxygen supply in the PCK induction period. The succinate concentration achieved 926.7mM with a yield of 0.91mol/mol during the anaerobic stage of fermentation in a 1.5-L reactor. qRT-PCR indicated that the two strategies enhanced transcription of genes related with glycerol metabolism and succinate production. Our results showed this metabolically engineered <I>E. coli</I> strain has a great potential in producing succinate using glycerol as carbon source.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Addition of acetate in the aerobic stage enhanced anaerobic succinate production. </LI> <LI> Aeration in the PCK induction stage affected the anaerobic metabolic capacities. </LI> <LI> 926.7mM of succinate was produced in the anaerobic stage in a 1.5-L bioreactor. </LI> <LI> The yield of succinate in the anaerobic stage achieved 0.91mol/mol. </LI> </UL> </P>

2017

Elsevier

publisher-specific-oa

0960-8524

231

pp.75-84

10.1016/j.biortech.2017.01.051

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77419848

http://manuscript.elsevier.com/S0960852417300883/pdf/S0960852417300883.pdf

Succinate; Glycerol; Acetate; Escherichia coli; Two-stage fermentation