Simultaneous glucose production from cellulose and fouling reduction using a magnetic responsive membrane reactor with superparamagnetic nanoparticles carrying cellulolytic enzymes
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Simultaneous glucose production from cellulose and fouling reduction using a magnetic responsive membrane reactor with superparamagnetic nanoparticles carrying cellulolytic enzymes
Gebreyohannes, Abaynesh Yihdego; Dharmjeet, Madhav; Swusten, Tom; Mertens, Matthias; Verspreet, Joran; Verbiest, Thierry; Courtin, Christophe M.; Vankelecom, Ivo F.J.
초록
<P><B>Abstract</B></P> <P>This work aimed at investigating simultaneous hydrolysis of cellulose and in-situ foulant degradation in a cellulose fed superparamagnetic biocatalytic membrane reactor (BMR<SUP>SP</SUP>). In this reactor, a dynamic layer of superparamagnetic bionanocomposites with immobilized cellulolytic enzymes were reversibly immobilized on superparamagnetic polymeric membrane using an external magnetic field.</P> <P>The formation of a dynamic layer of bionanocomposites on the membrane helped to prevent direct membrane-foulant interaction. Due to in-situ biocatalysis, there was limited filtration resistance. Simultaneous separation of the product helped to avoid enzyme product inhibition, achieve constant reaction rate over time and 50% higher enzyme efficiency than batch reactor.</P> <P>Stable enzyme immobilization and the ability to keep enzyme in the system for long period helped to achieve continuous productivity at very low enzyme but high solid loading, while also reducing the extent of membrane fouling. Hence, the BMR<SUP>SP</SUP> paves a path for sustainable production of bioethanol from the cheaply available lignocellulose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cellulolytic enzymes were immobilized on magnetic nanoparticles. </LI> <LI> The nanoparticles were reversibly immobilized on microporous membrane. </LI> <LI> Cellulose was continuously hydrolyzed at high solid and low enzyme loading. </LI> <LI> Constant productivity over time was achieved without enzyme product inhibition. </LI> <LI> The immobilized enzyme was efficient in in-situ membrane fouling degradation. </LI> </UL> </P>