Synthesis and modification of polyurethane for immobilization of Thermomyces lanuginosus (TLL) lipase for ethanolysis of fish oil in solvent free system
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Synthesis and modification of polyurethane for immobilization of Thermomyces lanuginosus (TLL) lipase for ethanolysis of fish oil in solvent free system
We report the synthesis of polyurethane (PU) with polyethylene glycol (PEG) by miniemulsion polymerization as technique to produce a support for enzyme immobilization and stabilization. In order to study the influence of molar weight and concentration of PEG in the enzyme@?support interaction, different PEG (400, 4000 and 6000Da) was used during the synthesis of PU@?PEG support to immobilize Thermomyces lanuginosus lipase (TLL). The enzymatic derivatives were effectively used in the production of polyunsaturated fatty acids (PUFAs), particularly derivative TLL@?PU@?PEG6000. This derivative showed the best results in terms of K<SUB>m</SUB> (0.183mM) and V<SUB>max</SUB> (45.79mmol/min/mL), calculated by Lineweaver@?Burk plot (double reciprocal plot) and catalytic efficient (250.22), 100% of yield immobilization and recovered activity, reaching the highest values to the ethyl esters production (260mM ethyl esters. U<SUP>@?1</SUP>) making this derivative a potential low cost catalyst for ethanolysis reactions in solvent free system.