BioChem - DOS

Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101

Bioprocess and biosystems engineering

Choi, Sun-A; Jung, Joo-Young; Kim, Kyochan; Kwon, Jong-Hee; Lee, Jin-Suk; Kim, Seung Wook; Park, Ji-Yeon; Yang, Ji-Won

<P>In this study, lipid extraction from Aurantiochytrium sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of Aurantiochytrium sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3% of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl36H2O showed a high lipid extraction yield (207.9 mg/g cell), when compared with that of [Emim]OAc, which was only 118.1 mg/g cell; notably however, when FeCl36H2O was mixed with [Emim]OAc (5:1, w/w), the yield was increased to 478.6 mg/g cell. When lipid was extracted by the FeCl36H2O/[Emim]OAc mixture at a 5:1 (w/w) blending ratio under 90 C, 30 min reaction conditions, the fatty acid content of the extracted lipid was a high purity 997.7 mg/g lipid, with most of the DHA having been extracted (30.2% of total fatty acids). Overall, lipid extraction from Aurantiochytrium sp. was enhanced by the synergistic effects of the molten-salt/ionic-liquid mixture with different ions.</P>

2014

Springer-Verlag

1615-7591

1615-7605

37

11

pp.2199-2204

10.1007/s00449-014-1197-2

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70445755