BioChem - DOS

Microbial oil produced from biodiesel by-products could enhance overall production

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Uckun Kiran, E.; Trzcinski, A.; Webb, C.

Glycerol and rapeseed meal, two major by-products of biodiesel production, have been tested for possible use as low-cost raw materials for the production of microbial bio-oil using the oleaginous yeast Rhodosporidium toruloides. Using fed-batch fermentation with crude glycerol and a novel nitrogen rich nutrient source derived from rapeseed meal as feed, it was shown that 13g/L lipids could be produced, compared with 9.4g/L when crude glycerol was used with yeast extract. When 100g/L pure glycerol was used, the final lipid concentration was 19.7g/L with the novel biomedium compared to 16.2g/L for yeast extract. The novel biomedium also resulted in higher lipid yields (0.19g lipid/g glycerol consumed compared to 0.12g/L) suggesting it provides a better carbon to nitrogen balance for accumulating lipids. FAMEs produced from the microbial lipids indicated a high degree of unsaturation confirming that the fatty acids produced from the novel biomedium have potential for biodiesel production.

2013

Elsevier Applied Science

0960-8524

129

pp.650-654

10.1016/j.biortech.2012.11.152

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65520773

http://eprints.usq.edu.au/28563/7/Glycerol%20paper%20%28Short%2918nov2012.pdf

Biodiesel; Microbial bio-oil; Rapeseed meal; Crude glycerol; Solid state fermentation