초록
<P><B>Highlights</B></P><P>► Explore feasible pollutant-degrading bacteria for biopolymer production. ► Disclose economically viable method for probing PHA-producing microbes. ► Unveil cradle-to-cradle strategy for materials recycling and wastewater treatment.</P> <P><B>Abstract</B></P><P>For eco-friendly recycling and reuses of biomaterials with sustainability, this feasibility study tended to use indigenous pollutant degrading bacteria for the production of biodegradable polymers – polyhydroxyalkanotes (PHAs) during wastewater treatment. First, feasible PHA-producing strains were qualitatively screened among pollutant-degrading microbes via Sudan black B staining (SB staining). Next, according to batch cultures using lauric acid as sole carbon source, the promising PHA-generating strains were obtained via comparative analysis upon the characteristics of cell growth and poly 3-hydroxybutyrate (PHB) production. <I>Aeromonas hydrophila</I> NIU01, YTl1, KB23 and <I>A. salmonicida</I> 741 were found to generate intracellular PHA content at higher levels of 19.35, 24.48, 22.52%, respectively. Plus, <I>Klebsiella pneumoniae</I> ZMd31, <I>Pseudomonas plecoglossicida</I> NIU-Y3 and <I>Chromobacterium violaceum</I> P1 produced lower PHA contents at 18.25, 5.37 and 4.17%, respectively.</P>