Enhancement of Fermentative Hydrogen Production in an Extreme-Thermophilic (70°C) Mixed-Culture Environment by Repeated Batch Cultivation

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논문

Enhancement of Fermentative Hydrogen Production in an Extreme-Thermophilic (70°C) Mixed-Culture Environment by Repeated Batch Cultivation

학술지

Current microbiology

저자명

Lu, Wenjing; Fan, Gaoyuan; Zhao, Chenxi; Wang, Hongtao; Chi, Zifang

초록

<P>Repeated batch cultivation was applied to enrich hydrogen fermentative microflora under extreme-thermophilic (70C) environment. Initial inoculums received from a hydrogen producing reactor fed with organic fraction of household solid wastes. In total seven transfers was conducted and maximum hydrogen yield reached 296 ml H(2)/g (2.38 mol/mol) glucose and 252 ml H(2)/g (2.03 mol/mol) for 1 and 2 g/l glucose medium, respectively. It was found that hydrogen production was firstly decreased and got increased gradually from third generation. Acetate was found to be the main metabolic by-product in all batch cultivation. Furthermore, the diversity of bacterial community got decreased after repeated batch cultivation. It was proved that repeated batch cultivation was a good method to enhance the hydrogen production by enriching the mixed cultures of dominant species.</P>

발행연도

2012

발행기관

Springer-Verlag

ISSN

0343-8651

ISSN

1432-0991

권

호

페이지

pp.427-432