초록
<P><B>Abstract</B></P><P>Inulin and glycerol were used as substrates for efficient erythritol and citric acid production by newly engineered<I>Yarrowia lipolytica</I>strains. Hydrolysis of inulin by the<I>Y. lipolytica</I>Wratislavia K1 strain was established by expressing the<I>Kluyveromyces marxianus INU1</I>gene. Erythritol was produced in two stages: inulin was used for biomass formation, followed by erythritol biosynthesis initiated by glycerol addition. The highest titer of erythritol obtained, 120.9 g L<SUP>−1</SUP>with the yield of 0.6 g g<SUP>−1</SUP>, was produced by the K1 INU 6 strain. Moreover, the K1 INU 6 strain in fed-batch culture produced a high amount of citric acid: 105.2 g L<SUP>−1</SUP>after 235 h from 200 g L<SUP>−1</SUP>of inulin. Maximum activity of inulinase during this culture was 14000 U g<SUP>−1</SUP>of cell dry mass. The presented study proves the potential of new<I>Y. lipolytica</I>transformants for efficient erythritol and citric acid production from inexpensive raw materials such as inulin and glycerol.</P>