초록
Defined co-cultures of hydrogen (H<SUB>2</SUB>) producers belonging to Citrobacter, Enterobacter, Klebsiella and Bacillus were used for enhancing the efficiency of biological H<SUB>2</SUB> production. Out of 11 co-cultures consisting of 2-4 strains, two co-cultures composed of Bacillus cereus EGU43, Enterobacter cloacae HPC123, and Klebsiella sp. HPC793 resulted in H<SUB>2</SUB> yield up to 3.0 mol mol<SUP>-1</SUP> of glucose. Up-scaling of the reactor by 16-fold resulted in a corresponding increase in H<SUB>2</SUB> production with an actual evolution of 7.44 L of H<SUB>2</SUB>. It constituted 58.2% of the total biogas. Continuous culture evolution of H<SUB>2</SUB> by co-cultures (B. cereus EGU43 and E. cloacae HPC123) immobilized on ligno-cellulosic materials resulted in 6.4-fold improvement in H<SUB>2</SUB> yield compared to free floating bacteria. This synergistic influence of B. cereus and E. cloacae can offer a better strategy for H<SUB>2</SUB> production than undefined or mixed cultures.