초록
<P><B>Highlights</B></P><P>► Detoxified sugarcane bagasse hydrolysate is alternative carbon source. ► DSCBH in peptone resulted in better biomass concentration than <SMALL>D</SMALL>-glucose. ► Lipid yield of <I>Yarrowia lipolytica</I> Po1g is promising for biodiesel production.</P> <P><B>Abstract</B></P><P>This study investigated the possibility of utilizing detoxified sugarcane bagasse hydrolysate (DSCBH) as an alternative carbon source to culture <I>Yarrowia lipolytica</I> Po1g for microbial oil and biodiesel production. Sugarcane bagasse hydrolysis with 2.5% HCl resulted in maximum total sugar concentration (21.38g/L) in which 13.59g/L is xylose, 3.98g/L is glucose, and 2.78g/L is arabinose. Detoxification of SCBH by Ca(OH)<SUB>2</SUB> neutralization reduced the concentration of 5-hydroxymethylfurfural and furfural by 21.31% and 24.84%, respectively. Growth of <I>Y. lipolytica</I> Po1g in DSCBH with peptone as the nitrogen source gave maximum biomass concentration (11.42g/L) compared to NH<SUB>4</SUB>NO<SUB>3</SUB> (6.49g/L). With peptone as the nitrogen source, DSCBH resulted in better biomass concentration than <SMALL>D</SMALL>-glucose (10.19g/L), <SMALL>D</SMALL>-xylose (9.89g/L) and NDSCBH (5.88g/L). The maximum lipid content, lipid yield and lipid productivity of <I>Y. lipolytica</I> Po1g grown in DSCBH and peptone was 58.5%, 6.68g/L and 1.76g/L-day, respectively.</P>