초록
<P><B>Abstract</B></P> <P>Herein, we report the secretome of <I>Penicillium chrysogenum</I> P33 under induction of lignocellulose for the first time. A total of 356 proteins were identified, including complete cellulases and numerous hemicellulases. Supplementing a commercial cellulase with increasing dosage of P33 enzyme cocktail from 1 to 5 mg/g substrate increased the release of reducing sugars from delignified corn stover by 21.4% to 106.8%. When 50% cellulase was replaced by P33 enzyme cocktail, release of reducing sugars was 78.6% higher than with cellulase alone. Meanwhile, glucan and xylan conversion was increased by 37% and 106%, respectively. P33 enzyme cocktail also enhanced commercial cellulase hydrolysis against four different delignified lignocellulosic biomass. These findings demonstrate that mixing appropriate amount of P33 cocktail with cellulase improves polysaccharide hydrolysis, suggesting P33 enzymes have great potential for industrial applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Penicillium chrysogenum</I> P33 secretes complete cellulases and numerous hemicellulases. </LI> <LI> A P33 enzyme cocktail acted synergistically with commercial cellulases. </LI> <LI> Replacement with P33 enzymes increased glucan and xylan conversion by 37% and 106% </LI> <LI> The P33 enzyme cocktail enhanced cellulase hydrolysis against different substrates. </LI> </UL> </P>