Enhanced saccharification of corn straw pretreated by alkali combining crude ligninolytic enzymes
Journal of chemical technology and biotechnology
Li, Jiang; Sun, Fenghui; Li, Xiangzhen; Yan, Zhiying; Yuan, Yuexiang; Liu, XiaoFeng
<P><B>Abstract</B></P><P><B>BACKGROUND:</B> This work investigated the monokaryogenesis of dikaryon strains of <I>Trametes hirsuta</I> by protoplasts regeneration for extracellular ligninolytic enzyme production. Saccharification of corn straw was enhanced by alkali pretreatment combining crude ligninolytic enzymes.</P><P><B>RESULTS:</B> Effectiveness of alkali pretreatment of corn straw on delignification was evaluated under different concentrations. About 45% lignin loss was achieved at the concentration of 1.5% NaOH. In addition, 79.0% sugar yield was obtained after combined pretreatment with NaOH and crude ligninolytic enzyme produced from monokaryotic strains of <I>Trametes hirsuta</I>. Scanning electron microscopy (SEM) images showed that the porosity and surface area increased significantly after combined pretreatment. The FTIR spectra indicated that great intensity changes occurred at the 890–900 cm<SUP>−1</SUP>, 1509–1513 cm<SUP>−1</SUP> and 1595 cm<SUP>−1</SUP> bands.</P><P><B>CONCLUSION:</B> The proposed combined pretreatment removes lignin and enhances saccharification of corn straw effectively. Copyright © 2012 Society of Chemical Industry</P>
2012
John Wiley Sons, Ltd.
0268-2575
1097-4660
87
12
pp.1687-1693
10.1002/jctb.3818
http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65073389
NaOH pretreatment; ligninolytic enzyme; delignification; sugar yield
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논문; 2012-05-10