초록
The effects of furans (furfural and 5-hydroxymethylfurfural (HMF)) on hydrogen (H<SUB>2</SUB>) production using mixed anaerobic cultures were evaluated by conducting batch experiments. Two mixed anaerobic cultures (culture A and B) fed furans plus glucose and treated with and without linoleic acid (LA) at pH 5.5 were maintained at 37 <SUP>o</SUP>C. In the LA inhibited cultures A and B fed 0.75 g L<SUP>-1</SUP> furfural and 0.25 g L<SUP>-1</SUP> HMF, the maximum H<SUB>2</SUB> yields observed were 1.89 +/- 0.27 mol mol<SUP>-1</SUP> glucose and 1.75 +/- 0.22 mol mol<SUP>-1</SUP> glucose, respectively. In cultures with maximum H<SUB>2</SUB> yields, Clostridium sp. and Flavobacterium sp. were dominant. Acetate, butyrate and ethanol were the major soluble metabolites detected in cultures A and B whereas propionate was also dominant in culture B. A canonical correspondence analysis based on the byproducts and the relative abundance of the terminal-restriction fragments revealed less variation between cultures treated with LA and low correlation value between the factors and the species composition.