Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant
학술지
International journal of hydrogen energy
저자명
Morra, S.; Arizzi, M.; Allegra, P.; La Licata, B.; Sagnelli, F.; Zitella, P.; Gilardi, G.; Valetti, F.
초록
[FeFe]-hydrogenases are the enzymes responsible for high yield H<SUB>2</SUB> production during dark fermentation in bio-hydrogen production plants. The culturable bacterial population present in a pilot-scale plant efficiently producing H<SUB>2</SUB> from waste materials was isolated, classified and identified by means of 16S rDNA gene analysis. The culturable part of the mixed population consists of nine bacterial species that include non-hydrogen producers (Lactobacillus, Enterococcus and Staphylococcus) and several Clostridium that are directly responsible for H<SUB>2</SUB> production. An extensive analysis of the expression of [FeFe]-hydrogenases in the three best producer strains was achieved by RT-PCR, covering the complete set of known genes for each species. This revealed that during H<SUB>2</SUB> production there are several different [FeFe]-hydrogenases simultaneously expressed, with genes belonging to the same phylogenetic and structural classification sharing similar transcriptional profiles.