초록
<P>Potato pulp, which is the industrial residue of potato processing for starch production, can be used for biochemicals production. In this study, a green, sustainable, highly productive technology for producing gluconic acid from potato pulp was developed. The cocktails of cellulases from <I>Trichoderma reesei</I> TX and <I>Penicillum oxalicum</I> JUA10–1 with commercial pectinase were prepared and used to hydrolyze hydrothermally treated potato pulp into fermentable sugars at a solids content of 25% (w/v). Eighty percent of the glucan in the potato pulp was able to be converted into glucose when 8 FPU/g of dry material (DM) of cellulase and 1000 PGU/g DM of pectinase were added. The enzymatic hydrolysates of the potato pulp were fermented into 81.4 g/L gluconic acid by <I>Gluconobacter oxidans</I> DSM 2003 for 20 h at 30 °C. The overall conversion yield from glucose to gluconic acid was 94.9%, and the productivity was 4.07 g/L/h, which was significantly better than that of previous studies. Finally, 546.48 g of gluconic acid was produced from per kilogram of dried potato pulp with this process.</P><P>An environmentally friendly method for the production of gluconic acid from sustainable agriculture waste via enzymatic hydrolysis was established.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ascecg/2017/ascecg.2017.5.issue-7/acssuschemeng.7b00992/production/images/medium/sc-2017-00992r_0007.gif'></P>