BioChem - DOS

Endocytosing Escherichia coli as a Whole-Cell Biocatalyst of Fatty Acids

ACS Synthetic biology

Shin, Jonghyeok; Yu, Jiwon; Park, Myungseo; Kim, Chakhee; Kim, Hooyeon; Park, Yunjeong; Ban, Choongjin; Seydametova, Emine; Song, Young-Ha; Shin, Chul-Soo; Chung, Kyung-Hwun; Woo, Ji-Min; Chung, Hyunwoo; Park, Jin-Byung; Kweon, Dae-Hyuk

<P>Whole cell biocatalysts can be used to convert fatty acids into various value-added products. However, fatty acid transport across cellular membranes into the cytosol of microbial cells limits substrate availability and impairs membrane integrity, which in turn decreases cell viability and bioconversion activity. Because these problems are associated with the mechanism of fatty acid transport through membranes, a whole-cell biocatalyst that can form caveolae-like structures was generated to promote substrate endocytosis. Caveolin-1 (<I>CAV1</I>) expression in <I>Escherichia coli</I> increased both the fatty acid transport rate and intracellular fatty acid concentrations <I>via</I> endocytosis of the supplemented substrate. Furthermore, fatty-acid endocytosis alleviated substrate cytotoxicity in <I>E. coli</I>. These traits attributed to bacterial endocytosis resulted in dramatically elevated biotransformation efficiencies in fed-batch and cell-recycle reaction systems when caveolae-forming <I>E. coli</I> was used for the bioconversion of ricinoleic acid (12-hydroxyoctadec-9-enoic acid) to (<I>Z</I>)-11-(heptanoyloxy) undec-9-enoic acid. We propose that <I>CAV1</I>-mediated endocytosing <I>E. coli</I> represents a versatile tool for the biotransformation of hydrophobic substrates.</P><BR>[FIG OMISSION]</BR>

2019

American Chemical Society

2161-5063

8

5

pp.1055-1066

10.1021/acssynbio.8b00519.s001

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART97799393

https://doi.org/10.1021/acssynbio.8b00519.s001

caveolin-1; heterologous caveolae; endocytosis; biotransformation; fatty acid