초록
<P><B>Abstract</B></P> <P>The process for non-axenic polyhydroxyalkanoate (PHA) production from organic waste generally comprises three steps: acidogenic fermentation of the waste stream, enrichment of a PHA-producing culture, and production of the PHA. This study assesses the feasibility of combining the enrichment and production step. Harvesting PHA-rich biomass directly from the sequencing batch reactor (SBR) used for enrichment of the microbial culture reduces capital cost, but may increase downstream-processing cost if the PHA content is significantly lowered. Operating an acetate-fed SBR at a volume exchange ratio of 0.75 (18h cycles, 1 d SRT) allowed the production of biomass with 70wt% poly(3-hydroxybutyrate) (PHB) in a single-step process. By increasing the exchange ratio to 0.83 (20h cycles) the PHB content of the harvested biomass increased to 75wt%, but the operational stability decreased. SBR operation at these high exchange ratios makes that bacteria have to increase their growth rate and external substrate is available for relatively long periods. This allows the establishment of larger flanking populations and negatively affected the kinetic properties of <I>Plasticicumulans acidivorans</I>, the predominant organism. Maximizing the volume exchange ratio is, therefore, a suitable strategy to produce large amounts of PHA in the SBR, but does not ensure the enrichment of a culture with superior PHA productivity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A one-stage process for simultaneous culture enrichment and PHB production. </LI> <LI> Production of up to 75wt% PHB from acetate in a non-axenic, single-step process. </LI> <LI> Cultivation at very high volume exchange ratios compromises PHB production rate. </LI> <LI> Increasing the volume exchange ratio forces bacteria to grow faster (at same SRT). </LI> </UL> </P>