BioChem - DOS

Production of eicosapentaenoic acid by Nannochloropsis oculata: Effects of carbon dioxide and glycerol

Journal of biotechnology

Shene, C.; Chisti, Y.; Vergara, D.; Burgos-Diaz, C.; Rubilar, M.; Bustamante, M.

The marine microalga Nannochloropsis oculata is a potential source of eicosapentaenoic acid (EPA, C20:5n3) and carotenoids for use in functional foods and nutraceuticals. Mixotrophic culture of N. oculata using glycerol was examined as a possible way of increasing the biomass and metabolite productivity relative to a pure photoautotrophic culture in modified f/2 medium. The effect of CO<SUB>2</SUB> supply was also tested. EPA production in semi-continuous culture with and without glycerol and CO<SUB>2</SUB> was evaluated. The effects of glycerol supplementation and light/dark cycling on the production of the biomass and EPA are reported for cultures conducted at a constant pH controlled using CO<SUB>2</SUB>. Consumption of glycerol was small, but its effects were significant. Glycerol enhanced the lipid content of the biomass but reduced the chlorophyll a content. Mixotrophic cultivation favored the production of lipids with a high percentage of saturated fatty acids that are generally desired in oils for making biodiesel. EPA concentration (5.3+/-0.6 to 27.5+/-1.6mg EPA/L) in N. oculata cultures depended strongly on growth conditions. The highest EPA concentration occurred in non-aerated mixotrophic culture with intermittent CO<SUB>2</SUB> supply without pH control. This EPA concentration (=27.5+/-1.6mg/L) was comparable to that obtained in semi-continuous culture without glycerol and pH control, and aerated with CO<SUB>2</SUB> enriched air during the light period (=23.6+/-1.1mg/L).

2016

Elsevier Science Publishers

0168-1656

1873-4863

239

pp.47-56

10.1016/j.jbiotec.2016.10.006

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76709179

Nannochloropsis oculata; Eicosapentaenoic acid; Polyunsaturated fatty acids; Lipids; Mixotrophic culture; Carotenoids