BioChem - DOS

Co-production of S-adenosyl-L-methionine and L-isoleucine in Corynebacterium glutamicum

Enzyme and microbial technology

Han, G.; Hu, X.; Wang, X.

In this study, production of S-adenosyl-L-methionine in Corynebacterium glutamicum was investigated by overexpressing genes metK and vgb. Compared with vector control, overexpression of metK alone in C. glutamicum ATCC13032 and IWJ001 increased SAM production 5.11 and 11.65 times, respectively; while overexpression of metK and vgb in C. glutamicum ATCC13032 and IWJ001 increased SAM production 5.83 and 14.95 times, respectively. Further studies on IWJ001/pDXW-8-metk-vgb showed that the limiting factor for SAM production is intracellular ATP supply. Since IWJ001 is an L-isoleucine production strain, IWJ001/pDXW-8-metk-vgb could produce both SAM and L-isoleucine. After 72h fermentation, SAM and L-isoleucine in IWJ001/pDXW-8-metk-vgb reached 0.67g/L and 13.8g/L, respectively. The results demonstrate the potential application of C. glutamicum for co-production of SAM and amino acids.

2015

IPC Science and Technology Press ; Elsevier Science Ltd

0141-0229

1879-0909

78

pp.27-33

10.1016/j.enzmictec.2015.06.003

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72438469

S-adenosyl-L-methionine; Methionine adenosyltransferase; L-methionine; L-isoleucine; Corynebacterium glutamicum