초록
<P><B>Highlights</B></P><P>► Sucrose-utilizing genes were expressed in <I>Escherichia coli</I> to enhance succinate production. ► The strain produced succinate from sucrose exceeding titers of other biocatalysts. ► We used mineral salt medium to produce succinate under simple anaerobic conditions. ► The fermentation conditions are expected to lower cost of succinate production. ► The costs of downstream processing and waste disposal would be also decreased.</P> <P><B>Abstract</B></P><P>Sucrose-utilizing genes (<I>cscKB</I> and <I>cscA</I>) from <I>Escherichia coli</I> KO11 were cloned and expressed in a metabolically engineered <I>E. coli</I> KJ122 to enhance succinate production from sucrose. KJ122 harboring a recombinant plasmid, pKJSUC, was screened for the efficient sucrose utilization by growth-based selection and adaptation. KJ122-pKJSUC-24T efficiently utilized sucrose in a low-cost medium to produce high succinate concentration with less accumulation of by-products. Succinate concentrations of 51g/L (productivity equal to 1.05g/L/h) were produced from sucrose in anaerobic bottles, and concentrations of 47g/L were produced in 10L bioreactor within 48h. Antibiotics had no effect on the succinate production by KJ122-pKJSUC-24T. In addition, succinate concentrations of 62g/L were produced from sugarcane molasses in anaerobic bottles, and concentrations of 56g/L in 10L bioreactor within 72h. These results demonstrated that KJ122-pKJSUC-24T would be a potential strain for bio-based succinate production from sucrose and sugarcane molasses.</P>