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Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

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논문

Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

학술지

RSC advances

저자명

Selvam, Kandasamy; Govarthanan, Muthusamy; Kamala-Kannan, Seralathan; Govindharaju, Munisamy; Senthilkumar, Balakrishnan; Selvankumar, Thangasamy; Sengottaiyan, Arumugam

초록

<P>The aim of this study was to assess the mixed combination of coffee pulp waste (CPW) and pineapple waste (PW) residues for cellulase production using newly isolated <I>Acinetobacter</I> sp. TSK-MASC in solid state fermentation. Response surface methodology based Box-Behnken design (BBD) was employed to optimize variables such as pH, incubation time, concentrations of CPW and PW. The BBD design investigation showed a sound adjustment of the quadratic model with the experimental statistics. Statistics based 3-D plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and culture parameters. The higher production (888 U mL<SUP>&#x2212;1</SUP>) was achieved after 60 h of incubation with 3.0 g L<SUP>&#x2212;1</SUP> of CPW and PW at pH 7.0.</P>

발행연도

2014

발행기관

The Royal Society of Chemistry

ISSN

2046-2069

4

25

페이지

pp.13045-13051

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1 2023-12-11

논문; 2014-01-01

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