BioChem - DOS

Monoseptic growth of fungal lipase producers under minimized sterile conditions: cultivation of Phialemonium curvatum in 350 L scale

Engineering in life sciences

Barig, Susann; Alisch, Romy; Nieland, Susanne; Wuttke, Anne; Grä ser, Yvonne; Huddar, Mahesh; Schnitzlein, Klaus; Stahmann, Klaus‐ Peter

<P><B>Abstract</B></P><P>A disadvantage of most microbial production processes is the need for sterile techniques. The objective of this study was the development of a robust fungal system allowing monoseptic growth with a minimum of sterile technique in plastic barrels. Selective growth conditions were achieved by mineral salts medium, known for the cultivation of <I>Botrytis cinerea</I>, but containing rapeseed oil instead of glucose as the sole source of carbon and energy. Furthermore, pH 3 was adjusted. A screening of fungi suitable for that system revealed <I>Phialemonium curvatum</I> AW02 isolated from compost. <I>P. curvatum</I> AW02 was superior in comparison with four further fungal isolates because high titers of hydrophilic spores were found in submerged production. Second, a biofilm formation on plastic segments or moving beds made harvesting of the biomass comfortable. Cultivations with volumes of 100 or 350&thinsp;L showed no contaminations by bacteria when all conditions were controlled. Two independent approaches showed the dependance of growth on lipases in the cultivation system. A <I>B. cinerea</I> strain knocked out in <I>lip1</I> showed reduced growth in comparison to the wild type because the first catabolic step is the triglyceride hydrolysis. <I>P. curvatum</I> AW02 lipase activity was detected. More than 90% was found to be cell wall associated. Solid shear stress liberated two active proteins showing IEPs of 4.7 or 5.6.</P>

2011

WILEY&#x2010;VCH Verlag

1618-0240

1618-2863

11

4

pp.387-394

10.1002/elsc.201000219

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57263905

Botrytis cinerea; Geotrichum candidum; Mucor circinelloides; Pseudozyma sp.; Torrubiella sp.