BioChem - DOS

Sweet-and-salty biocatalysis: Fructooligosaccharides production using Cladosporium cladosporioides in seawater

Process biochemistry

Zambelli, P.; Serra, I.; Fernandez-Arrojo, L.; Plou, F.J.; Tamborini, L.; Conti, P.; Contente, M.L.; Molinari, F.; Romano, D.

Production of fructooligosaccharides (FOS) from sucrose was obtained using a bioprocess entirely performed in seawater. The halophilic fungus Cladosporium cladosporioides MUT 5506 was grown in a seawater-based medium and mycelium displayed an optimal activity in seawater at 50-60<SUP>o</SUP>C, being stable up to 60<SUP>o</SUP>C. Under optimized conditions in seawater (50<SUP>o</SUP>C, sucrose 600g/L, lyophilized mycelium 40g/L), C. cladosporioides gave a maximum FOS yield of 344g/L after 72h with a preponderance of <SUP>1</SUP>F-FOS derivatives (1-kestose 184g/L, 1-nystose 98g/L and 1-fructofuranosylnystose 22g/L), and the noteworthy presence of the non-conventional disaccharide blastose (30g/L after 144h). Lyophilized mycelium exhibited good stability in seawater (76% of the initial activity was retained after 15 cycles of reutilization). This proof-of-concept application reports for the first time the production of FOS in a non-conventional medium such as seawater.

2015

Elsevier Applied Science

1359-5113

50

7

pp.1086-1090

10.1016/j.procbio.2015.04.006

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72224192

https://air.unimi.it/bitstream/2434/276176/6/revised_FOS%20mare.pdf

Fructooligosaccharides; Fructofuranosidase; Cladosporium cladosporioides; Seawater; Biocatalysis