BioChem - DOS

Enhancing the growth rate and astaxanthin yield of Haematococcus pluvialis by nuclear irradiation and high concentration of carbon dioxide stress

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Cheng, J.; Li, K.; Yang, Z.; Zhou, J.; Cen, K.

Unicellular green microalgae Haematococcus pluvialis was mutated with <SUP>60</SUP>Co-&gamma; irradiation to promote growth rate and increase astaxanthin yield under high concentration of CO<SUB>2</SUB> stress. The average specific growth rate of H. pluvialis mutated with 4000Gy &gamma;-ray irradiation was increased by 15% compared with the original strain with air aeration. The mutant grew best with 6% CO<SUB>2</SUB> (the maximum specific growth rate was 0.60/d) when it was cultured with high concentrations of CO<SUB>2</SUB> (2-10%). The peak biomass productivity (0.16g/L/d) of the mutant cultured with 6% CO<SUB>2</SUB> was 82% higher than that of the mutant with air. The astaxanthin yield and lipid content of the mutant induced with 6% CO<SUB>2</SUB> and high light (108&mu;molphotonsm<SUP>-2</SUP>s<SUP>-1</SUP>) increased to 46.0mg/L and 45.9%, which were 2.4 and 1.3 times higher than those of the wild-type strain, respectively.

2016

Elsevier Applied Science

0960-8524

204

pp.49-54

10.1016/j.biortech.2015.12.076

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74856737

Haematococcus pluvialis; Nuclear mutagenesis; High CO2; Astaxanthin; Growth rate