BioChem - DOS

High biobutanol production integrated with in situ extraction in the presence of Tween 80 by Clostridium acetobutylicum

Process biochemistry

Xin, Fengxue; Liu, Jie; He, Mingxiong; Wu, Bo; Ni, Yufan; Dong, Weiliang; Zhang, Wenming; Hu, Guoquan; Jiang, Min

<P><B>Abstract</B></P> <P>This study investigates the improvement of butanol production in the presence &ndash; Tween 80 by <I>Clostridium acetobutylicum</I> NJ4. With supplementation of 15% Tween 80 (v/v), the final butanol titer could be significantly enhanced to 18.65 g/L from 70 g/L glucose, which is 38% higher than that in the control batch (13.47 g/L). With further <I>in situ</I> extraction using biodiesel, the butanol titer was finally improved to 33.90 g/L in the fed batch mode. The supplementation of Tween 80 could improve butanol tolerance of strain NJ4 up to 18 g/L compared to 10 g/L of control. Meanwhile, butanol dehydrogenase activity was improved to 3.89 U/mg, which is 80.93% higher than that of the control. These factors may attribute to the high butanol production. The finding of this study thus offers fundamental knowledge for the future development of alternative ABE production strategies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The butanol titer was enhanced to 18.65 g/L in the presence of 15% Tween 80. </LI> <LI> Tween 80 could significantly improve butanol tolerance up to 18 g/L. </LI> <LI> Tween 80 could improve butanol dehydrogenase activities. </LI> <LI> <I>In situ</I> extraction using biodiesel gave a high butanol production of 33.90 g/L. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2018

Elsevier

1359-5113

67

pp.113-117

10.1016/j.procbio.2018.01.013

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART81670112

Biobutanol; Non-ionic surfactant; In situ removal; Tolerance fed batch fermentation