BioChem - DOS

Advancement of green process through microwave-assisted extraction of bioactive metabolites from Arthrospira platensis and bioactivity evaluation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Esquivel-Herná ndez, Diego A.; Rodrí guez-Rodrí guez, José Rostro-Alanis, Magdalena; Cué llar-Bermú dez, Sara P.; Mancera-Andrade, Elena I.; Nú ñ ez-Echevarrí a, Jade E.; Garcí a-Pé rez, J. Saú l; Chandra, Rashmi; Parra-Saldí var, Roberto

<P><B>Abstract</B></P> <P>Bioactivity and functional properties of cyanobacterial extract mostly depends on process of extraction, temperature and solvent used (polar or non-polar). To evaluate these parameters a design of experiment (DOE; using a 2<SUP>k</SUP> design) was performed with <I>Arthrospira platensis.</I> Extraction process was optimized through microwave-assisted extraction considering solvent ratio, temperature and time of extraction with polar (PS) and non-polar (NPS). Maximum extract yield obtained was 4.32&plusmn;0.25% and 5.26&plusmn;0.11% (w/w) respectively for PS and NPS. Maximum content of bioactive metabolites in PS extracts were thiamine (846.57&plusmn;14.12&mu;g/g), riboflavin (101.09&plusmn;1.63&mu;g/g), C-phycocyanin (2.28&plusmn;0.10&mu;g/g) and A-phycocyanin (4.11&plusmn;0.03&mu;g/g), while for NPS extracts were &alpha;-tocopherol (37.86&plusmn;0.78&mu;g/g), &beta;-carotene (123.64&plusmn;1.45&mu;g/g) and 19.44&plusmn;0.21mg/g of fatty acids. <I>A. platensis</I> PS extracts showed high antimicrobial activity and PS extracts had antioxidant activity of 0.79&plusmn;0.12&mu;molTE/g for FRAP assay, while for NPS extracts 1.03&plusmn;0.08&mu;mol &alpha;-TE/g for FRAP assay.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Non polar solvent have higher extract yield. </LI> <LI> Use of green solvent offer biologically active cyanobacterial extract. </LI> <LI> Microwave-assisted extraction for high reproducibility and purity of extract. </LI> <LI> High levels of solvent ratio, temperature and time result in high extract yield. </LI> <LI> Polar solvent extract shows higher antibacterial activity. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2017

Elsevier

0960-8524

224

pp.618-629

10.1016/j.biortech.2016.10.061

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77034361

Microwave-assisted extraction; β-Carotene; α-Tocopherol; γ-Linolenic acid; Phycocyanin; FRAP