초록
<P><B>Abstract</B></P> <P>Commercial fructo-oligosaccharides (FOS) are predominantly produced from sucrose by transfructosylation process that presents a maximum theoretical yield below 0.60g<SUB>FOS</SUB> g<SUB>Sucrose</SUB> <SUP>−1</SUP>. To obtain high-content FOS, costly purification is generally employed. Additionally, high-content FOS can be produced from inulin by using endo-inulinases. However, commercial endo-inulinases have not been extensively used in scale-up production of FOS. In the present study, a one-step bioprocess that integrated endo-inulinase production, FOS fermentation, and non-FOS sugars removal into one reactor was proposed to produce high-content FOS from inulin. The bioprocess was implemented by a recombinant yeast strain JZHΔS-TSC, in which a heterologous endo-inulinase gene was expressed and the inherent invertase gene <I>SUC2</I> was disrupted. FOS fermentation at 40°C from 200g/L chicory inulin presented the maximun titer, yield, and productivity of 180.2±0.8g/L, 0.9g<SUB>FOS</SUB> g<SUB>Inulin</SUB> <SUP>−1</SUP>, and 7.51±0.03g/L/h, respectively. This study demonstrated that the one-step bioprocess was simple and highly efficient.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Saccharomyces cerevisiae</I> was used to produce fructo-oligosaccharides (FOS). </LI> <LI> The <I>SUC2</I> gene was disrupted and an endo-inulinase gene was expressed in <I>S. cerevsiae</I>. </LI> <LI> A highest yield of 0.9g<SUB>FOS</SUB> g<SUB>Inulin</SUB> <SUP>−1</SUP> was achieved by fermentation at 40°C. </LI> <LI> A simple and efficient one-step bioprocess was established. </LI> </UL> </P>