BioChem - DOS

13C-metabolic flux analysis in S-adenosyl-L-methionine production by Saccharomyces cerevisiae

Journal of bioscience and bioengineering

Hayakawa, K.; Kajihata, S.; Matsuda, F.; Shimizu, H.

S-Adenosyl-l-methionine (SAM) is a major biological methyl group donor, and is used as a nutritional supplement and prescription drug. Yeast is used for the industrial production of SAM owing to its high intracellular SAM concentrations. To determine the regulation mechanisms responsible for such high SAM production, <SUP>13</SUP>C-metabolic flux analysis (<SUP>13</SUP>C-MFA) was conducted to compare the flux distributions in the central metabolism between Kyokai no. 6 (high SAM-producing) and S288C (control) strains. <SUP>13</SUP>C-MFA showed that the levels of tricarboxylic acid (TCA) cycle flux in SAM-overproducing strain were considerably increased compared to those in the S228C strain. Analysis of ATP balance also showed that a larger amount of excess ATP was produced in the Kyokai 6 strain because of increased oxidative phosphorylation. These results suggest that high SAM production in Kyokai 6 strains could be attributed to enhanced ATP regeneration with high TCA cycle fluxes and respiration activity. Thus, maintaining high respiration efficiency during cultivation is important for improving SAM production.

2015

Society for Bioscience and Bioengineering, Japan ; Distributed outside Japan by Elsevier Science

1389-1723

1347-4421

120

5

pp.532-538

10.1016/j.jbiosc.2015.03.010

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART73233531

13C-based metabolic flux analysis; Crabtree effect; Central carbon metabolism; Redox balance; Saccharomyces cerevisiae