BioChem - DOS

Development of a mathematical model for the growth associated polyhydroxybutyrate fermentation by Azohydromonas australica and its use for the design of fed-batch cultivation strategies

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Gahlawat, G.; Srivastava, A.K.

In the present investigation, batch cultivation of Azohydromonas australica DSM 1124 was carried out in a bioreactor for growth associated PHB production. The observed batch PHB production kinetics data was then used for the development of a mathematical model which adequately described the substrate limitation and inhibition during the cultivation. The statistical validity test demonstrated that the proposed mathematical model predictions were significant at 99% confidence level. The model was thereafter extrapolated to fed-batch to identify various nutrients feeding regimes during the bioreactor cultivation to improve the PHB accumulation. The distinct capability of the mathematical model to predict highly dynamic fed-batch cultivation strategies was demonstrated by experimental implementation of two fed-batch cultivation strategies. A significantly high PHB concentration of 22.65g/L & an overall PHB content of 76% was achieved during constant feed rate fed-batch cultivation which is the highest PHB content reported so far using A. australica.

2013

Elsevier Applied Science

0960-8524

137

pp.98-105

10.1016/j.biortech.2013.03.023

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65929531

Polyhydroxybutyrate; Azohydromonas australica; Modeling; Process parameters; Fed-batch culture