BioChem - DOS

Low-cost production of 6G-fructofuranosidase with high value-added astaxanthin by Xanthophyllomyces dendrorhous

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Ning, Yawei; Li, Qiang; Chen, Feng; Yang, Na; Jin, Zhengyu; Xu, Xueming

<P><B>Highlights</B></P><P>&#x025BA; The production of <SUP>6</SUP>G-FFase with value-added astaxanthin was examined. &#x025BA; Using CSL as nitrogen source significantly reduced the production cost of <SUP>6</SUP>G-FFase. &#x025BA; The yield of <SUP>6</SUP>G-FFase and the productivity of neo-FOS were relatively high. &#x025BA; The medium cost reduced 66.3% compared with that before optimization. &#x025BA; This medium could make the neo-FOS production process waste-free.</P> <P><B>Abstract</B></P><P>The effects of medium composition and culture conditions on the production of <SUP>6</SUP>G-fructofuranosidase with value-added astaxanthin were investigated to reduce the capital cost of neo-fructooligosaccharides (neo-FOS) production by <I>Xanthophyllomyces dendrorhous</I>. The sucrose and corn steep liquor (CSL) were found to be the optimal carbon source and nitrogen source, respectively. CSL and initial pH were selected as the critical factors using Plackett&ndash;Burman design. Maximum <SUP>6</SUP>G-fructofuranosidase 242.57U/mL with 5.23mg/L value-added astaxanthin was obtained at CSL 52.5mL/L and pH 7.89 by central composite design. Neo-FOS yield could reach 238.12g/L under the optimized medium conditions. Cost analysis suggested 66.3% of substrate cost was reduced compared with that before optimization. These results demonstrated that the optimized medium and culture conditions could significantly enhance the production of <SUP>6</SUP>G-fructofuranosidase with value-added astaxanthin and remarkably decrease the substrate cost, which opened up possibilities to produce neo-FOS industrially.</P>

2012

0960-8524

104

pp.660-667

10.1016/j.biortech.2011.10.098

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58196852

6G-fructofuranosidase; Medium optimization; Xanthophyllomyces dendrorhous; Corn steep liquor; Neo-FOS