초록
<P><B>Abstract</B></P> <P>In our previous research, two-stage anaerobic co-fermentation (TSAC) treating lipid-extracted microalgae waste (LEMW) mixed with food waste leachate (FWL) was successfully designed to recover the additional energy (H<SUB>2</SUB> and CH<SUB>4</SUB>) while mitigating the inhibition of leftover chloroform (CF). This study aimed to elucidate the change in the microbial community and their association with the CF degradation in the TSAC. 90% of CF was dechlorinated in the acidogenic reactor of TSAC when LEMW was fed with FWL at a ratio of 40:60. The results of microbial analysis clearly showed that <I>Dehalobacter</I> (5.3%) played the role in dehalorespiration while <I>Clostridium</I> (8.9%) and <I>Enterobacter</I> (3.6%) promoted cometabolic dechlorination. In contrast, the microbial consortia were completely inhibited by CF when LEMW was fed without FWL. In the methanogenic reactor of TSAC, increased <I>Methanosarcina</I> (57.8%), <I>Methanobacterium</I> (10.4%), and <I>Methanosaeta</I> (8.6%) were observed. Those were involved in successful conversion of acetate and H<SUB>2</SUB> into CH<SUB>4</SUB>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Changes of microbial consortia associated with dechlorination of leftover CF. </LI> <LI> 90% of CF was dechlorinated in the acidogenic stage fed by LEWM: FWL (40:60). </LI> <LI> CF removal by <I>Dehalobacter</I>, <I>Clostridium</I>, and <I>Enterobacter</I> in the acidogenic stage. </LI> <LI> High abundance of <I>Methanosarcina</I> in the methanogenic stage. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>