BioChem - DOS

Online Capillary Electrophoresis for Monitoring Carboxylic Acid Production by Yeast during Bioreactor Cultivations

Analytical chemistry

Turkia, Heidi; Holmströ m, Sami; Paasikallio, Toni; Siré n, Heli; Penttilä , Merja; Pitkä nen, Juha-Pekka

<P>Bioprocess monitoring can improve the understanding and control of biotechnological processes. When analyses are carried out as automated online measurements, manual steps of the analysis procedures are avoided, thus decreasing both the time required for analyses and systematic errors. In this study, an online capillary electrophoresis (CE) system with flow-through sample vial made in-house and action control programming was assembled to monitor carboxylic acid production by <I>Kluyveromyces lactis</I> and <I>Saccharomyces cerevisiae</I> during two different bioreactor cultivations. The relative standard deviations were less than 0.6% for intraday migration times and the total analysis time was less than 20 min. The system operated continuously and automatically up to 6 days and produced data concerning carboxylic acid production during the cultivations. The successful test runs demonstrated that this system has potential for the monitoring of biotechnological processes.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-20/ac402113x/production/images/medium/ac-2013-02113x_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac402113x'>ACS Electronic Supporting Info</A></P>

2013

American Chemical Society

0003-2700

1520-6882

85

20

pp.9705-9712

10.1021/ac402113x

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART67189813