BioChem - DOS

Evaluation of activity of Bacillus lipase (free and immobilized) treated with compressed propane

Journal of molecular catalysis. B, Enzymatic

Carvalho, N.B.; Silva, M.A.d.O.; Fricks, A.T.; Franceschi, E.; Dariva, C.; Zanin, G.M.; Lima, A.S.; Soares, C.M.F.

This work is focused on the evaluation the lipase stability from newly isolated Bacillus sp. ITP-001 after different times treatment with propane at 45 and 60<SUP>o</SUP>C and pressures of 50 and 200bar, considering the free form of lipase and also the enzyme immobilized by the sol-gel technique. The hydrolytic activity of the free lipase remained constant up 360min of incubation at 60<SUP>o</SUP>C/200bar (approximately 15% of constant activity gain). For the esterification reaction a gain up to 500% was observed at the initial time when the enzyme was submitted to 45<SUP>o</SUP>C/200bar. For the immobilized enzyme, a lose in the hydrolytic activity was observed after the high pressure treatment, but the esterification activity was improved up to 50% at 60<SUP>o</SUP>C/200bar. In general, these results show that enzyme activity changes significantly depending on the enzyme and the experimental conditions investigated.

2014

Elsevier

1381-1177

1873-3158

99

pp.130-135

10.1016/j.molcatb.2013.11.006

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68282680

Compressed propane; Enzyme activity; Bacillus lipase; Immobilization