BioChem - DOS

Facile enzymatic synthesis of sugar 1-phosphates as substrates for phosphorylases using anomeric kinases

Carbohydrate research

Liu, Yuan; Nishimoto, Mamoru; Kitaoka, Motomitsu

<P><B>Abstract</B></P> <P>Three sugar 1-phosphates that are donor substrates for phosphorylases were produced at the gram scale from phosphoenolpyruvic acid and the corresponding sugars by the combined action of pyruvate kinase and the corresponding anomeric kinases in good yields. These sugar 1-phosphates were purified through two electrodialysis steps. &alpha;-<SMALL>D</SMALL>-Galactose 1-phosphate was finally isolated as crystals of dipotassium salts. &alpha;-<SMALL>D</SMALL>-Mannose 1-phosphate and 2-acetamido-2-deoxy-&alpha;-<SMALL>D</SMALL>-glucose 1-phosphate were isolated as crystals of bis(cyclohexylammonium) salts.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We describe one-pot enzymatic synthesis of three sugar 1-phosphates. </LI> <LI> Anomeric kinases were used in the presence of an ATP-regeneration system. </LI> <LI> These sugar 1-phosphates were available as crystals of particular salts. </LI> <LI> These sugar 1-phosphates are donor substrates for phosphorylases. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2015

Elsevier

0008-6215

1873-426x

401

pp.1-4

10.1016/j.carres.2014.10.014

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71067823

Sugar 1-phosphate; Anomeric kinase; Pyruvate kinase; Electrodialysis; Substrate for phosphorylase