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Genome-Wide Analysis of the Polygalacturonase Gene Family Sheds Light on the Characteristics, Evolutionary History, and Putative Function of Akebia trifoliata

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논문

Genome-Wide Analysis of the Polygalacturonase Gene Family Sheds Light on the Characteristics, Evolutionary History, and Putative Function of Akebia trifoliata

학술지

International journal of molecular sciences

저자명

Yi, Xiaoxiao; Chen, Wei; Guan, Ju; Zhu, Jun; Zhang, Qiuyi; Yang, Huai; Yang, Hao; Zhong, Shengfu; Chen, Chen; Tan, Feiquan; Ren, Tianheng; Luo, Peigao

초록

<P>Polygalacturonase (PG) is one of the largest families of hydrolytic enzymes in plants. It is involved in the breakdown of pectin in the plant cell wall and even contributes to peel cracks. Here, we characterize <I>PGs</I> and outline their expression profiles using the available reference genome and transcriptome of <I>Akebia trifoliata</I>. The average length and exon number of the 47 identified <I>AktPGs,</I> unevenly assigned on 14 chromosomes and two unassembled contigs, were 5399 bp and 7, respectively. The phylogenetic tree of 191 <I>PGs</I>, including 47, 57, 51, and 36 from <I>A. trifoliata</I>, <I>Durio zibethinus</I>, <I>Actinidia chinensis,</I> and <I>Vitis vinifera</I>, respectively, showed that <I>AktPGs</I> were distributed in all groups except group G and that 10 <I>AktPGs</I> in group E were older, while the remaining 37 <I>AktPGs</I> were younger. Evolutionarily, all <I>AktPGs</I> generally experienced whole-genome duplication (WGD)/segmental repeats and purifying selection. Additionally, the origin of conserved domain III was possibly associated with a histidine residue (H) substitute in motif 8. The results of both the phylogenetic tree and expression profiling indicated that five <I>AktPGs</I>, especially <I>AktPG25,</I> could be associated with the cracking process. Detailed information and data on the <I>PG</I> family are beneficial for further study of the postharvest biology of <I>A. trifoliata</I>.</P>

발행연도

2023

발행기관

MDPI

ISSN

1661-6596

ISSN

1422-0067

24

23

페이지

pp.16973

주제어

Akebia trifoliata; fruit cracking; polygalacturonase; evolutionary relationship; expression profiling

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1 2023-12-11

논문; 2023-11-30

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