초록
<P><B>Abstract</B></P> <P>Acetone-butanol-ethanol (ABE) fermentation from food processing waste is one way to reduce cost. In this study, corncob hydrolysate and corn steep liquor (CSL), two waste materials from corn processing industries, were used as carbon and nitrogen sources to yield butanol by using <I>Clostridium beijerinckii</I> SE-2. Media compositions favoring butanol production were investigated using statistical experimental designs. Media components were first screened using a fractional factorial experimental design. CSL and CH<SUB>3</SUB>COONH<SUB>4</SUB> were found to be the significant variables among six factors, including the contents of CSL, CH<SUB>3</SUB>COONH<SUB>4</SUB>, K<SUB>2</SUB>HPO<SUB>4</SUB>-KH<SUB>2</SUB>PO<SUB>4</SUB>, MnSO<SUB>4</SUB> · H<SUB>2</SUB>O, MgSO<SUB>4</SUB> · 7H<SUB>2</SUB>O and FeSO<SUB>4</SUB> · 7H<SUB>2</SUB>O. The two factors were further optimized by the steepest assent and central composite rotatable design. The validated experiments showed that the total ABE in the system was 19.22 g L<SUP>−1</SUP> and the concentration of butanol could reach 11.65 g L<SUP>−1</SUP> with the optimized medium, which was 42% higher than the initial medium. Scale-up fermentation with optimized medium in the 100 L bioreactor resulted in 20.29 g L<SUP>−1</SUP> of ABE and 11.92 g L<SUP>−1</SUP> of butanol. Moreover, <I>C. beijerinckii</I> SE-2 can use both glucose and xylose from corncob hydrolysate. In conclusion, our study suggested that the waste of the corn processing industry can be used as sources to produce butanol by <I>Clostridium,</I> and statistical experimental designs are a useful approach for optimizing media compositions for butanol production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Corncob hydrolysate and corn steep liquor were used as carbon and nitrogen sources to yield butanol. </LI> <LI> <I>C. beijerinckii</I> SE-2 can use both glucose and xylose from corncob hydrolysate. </LI> <LI> Three statistical experimental designs were used to increase the production of butanol. </LI> <LI> The production of butanol increased 42% after optimization. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>