A novel production process for optically pure L-lactic acid from kitchen refuse using a bacterial consortium at high temperatures

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- 바이오정밀화학
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  2. 기타
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- 의료용 화학소재
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  2. 식품첨가제

논문

A novel production process for optically pure L-lactic acid from kitchen refuse using a bacterial consortium at high temperatures

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Tashiro, Yukihiro; Matsumoto, Hiroko; Miyamoto, Hirokuni; Okugawa, Yuki; Pramod, Poudel; Miyamoto, Hisashi; Sakai, Kenji

초록

Abstract We investigated L-lactic acid production in static batch fermentation of kitchen refuse using a bacterial consortium from marine-animal-resource (MAR) composts at temperatures ranging from 30 to 65°C. At relatively low temperatures butyric acid accumulated, whereas at higher temperatures L-lactic acid was produced. In particular, fermentation at 50°C produced 34.5gL−1 L-lactic acid with 90% lactic acid selectivity and 100% optical purity. Denaturing gradient gel electrophoresis indicated that dominant bacteria present in the original MAR composts diminished rapidly and Bacillus coagulans strains became the dominant contributors to L-lactic acid production at 45, 50 and 55°C. This is the first report of the achievement of 100% optical purity of L-lactic acid using a bacterial consortium. Highlights <UL> <LI> We achieved selective L-lactic acid fermentation using MAR composts. </LI> <LI> The results of this study suggest the new academic concept of meta-fermentation. </LI> <LI> Bacillus coagulans was the main producer of L-lactic acid in meta-fermentation. </LI> </UL>

발행연도

2013

발행기관

Elsevier

ISSN

0960-8524

권

146

페이지

pp.672-681

DOI

10.1016/j.biortech.2013.07.102