Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars
학술지
Applied and environmental microbiology
저자명
Groff, Dan; Benke, Peter I.; Batth, Tanveer S.; Bokinsky, Gregory; Petzold, Christopher J.; Adams, Paul D.; Keasling, Jay D.
초록
<P><B>ABSTRACT</B><P>Escherichia coli has the potential to be a powerful biocatalyst for the conversion of lignocellulosic biomass into useful materials such as biofuels and polymers. One important challenge in using E. coli for the transformation of biomass sugars is diauxie, or sequential utilization of different types of sugars. We demonstrate that, by increasing the intracellular levels of the transcription factor XylR, the preferential consumption of arabinose before xylose can be eliminated. In addition, XylR augmentation must be finely tuned for robust coutilization of these two hemicellulosic sugars. Using a novel technique for scarless gene insertion, an additional copy of <I>xylR</I> was inserted into the <I>araBAD</I> operon. The resulting strain was superior at cometabolizing mixtures of arabinose and xylose and was able to produce at least 36% more ethanol than wild-type strains. This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars. </P></P>