초록
<P><B>Background</B></P><P>Dihydrodipicolinate synthase (DHDPS) is an allosteric enzyme, which catalyzes the first unique step of lysine biosynthesis in prokaryotes, higher plants and some fungi. To date, the biological roles of DHDPS in filamentous fungi are poorly understood.</P><P><B>Results</B></P><P>In this study, on the basis of comparative genome resequencing, a DHDPS gene was found to be specific in <I>Fusarium asiaticum</I>, named <I>FaDHDPS1,</I> which showed high amino acid identity to that of entomopathogenic fungus. Subcellular localization of the FaDHDPS1-GFP fusion protein was mainly concentrated in the cytoplasm of conidia and dispersed in the cytoplasm during conidial germination. To reveal the biological functions, both deletion and complementation mutants of <I>FaDHDPS1</I> were generated. The results showed that the <I>FaDHDPS1</I> deletion mutant was defective in conidiation, virulence and DON biosynthesis. In addition, deletion of <I>FaDHDPS1</I> resulted in tolerance to sodium pyruvate, lysine, low temperature and Congo red.</P><P><B>Conclusion</B></P><P>Results of this study indicate that <I>FaDHDPS1</I> plays an important role in the regulation of vegetative differentiation, pathogenesis and adaption to multiple stresses in <I>F. asiaticum</I>.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1186/s12866-018-1268-7) contains supplementary material, which is available to authorized users.</P>