초록
<P><B>ABSTRACT</B></P><P>WAVE™ rocking disposable bioreactors have been successfully utilized for bioproduct development from bacteria, yeast, microalgae, and animal and plant cells but not from cyanobacteria so far. N<SUB>2</SUB>‐fixing cyanobacteria represent a prolific bioproducts source with reduced cultivation costs. In this study, 1 L cultures of the N<SUB>2</SUB>‐fixing cyanobacterium <I>Anabaena siamensis</I> grown diazotrophically in the WAVE™ bioreactor exhibited increased phosphate consumption and 37–70% higher CO<SUB>2</SUB> fixation rates than those grown in conventional bubbled suspension (BS) batch cultures. This generated 40–80% increased biomass productivities in the WAVE™ bioreactor reaching 60 mg L<SUP>−1</SUP> day<SUP>−1</SUP> when supplemented with 10% CO<SUB>2</SUB>. Consequently, WAVE™ generated 36–153% more protein, lipid, and carbohydrate than BS, including 47–100% increased productivity of phycocyanin and stearidonic acid (SA) with relevant biomedical applications. While the type of culture system (BS or WAVE<SUP>TM</SUP>) did not affect the biochemical profile of cyanobacterial biomass, 10% CO<SUB>2</SUB> supplementation induced a significant decrease in fatty acids and phycocyanin contents (mg g<SUP>−1</SUP> DW). Therefore, for commercial applications, the CO<SUB>2</SUB> supplementation of WAVE™ should be optimized for each targeted bioproduct separately. This study opens possibilities for upgrading the WAVE™ systems to photobioreactors (PBRs) for bioproduct development from cyanobacteria, with opportunities and challenges critically evaluated herein. Biotechnol. Bioeng. 2015;112: 621–626. © 2014 Wiley Periodicals, Inc.</P>