초록
<P><B>Abstract</B></P> <P>Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from <I>Palmaria palmata</I> was investigated for the first time. In engineered <I>Escherichia coli</I> KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase–acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20±0.02mol/mol) than glucose (0.48±0.03mol/mol). The concentration and molar yield of succinic acid were 22.40±0.12g/L and 1.13±0.02mol/mol total sugar respectively after 72h dual phase fermentation from <I>P. palmata</I> hydrolysate which composed of glucose (12.57±0.17g/L) and galactose (18.03±0.10g/L). The results demonstrate that <I>P. palmata</I> red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel biomass (<I>P. palmata</I>) was used for the production of succinic acid. </LI> <LI> <I>E. coli</I> KLPPP was constructed to produce succinic acid from <I>P. palmata</I> hydrolysate. </LI> <LI> <I>E. coli</I> KLPPP exhibited higher molar yield of succinic acid on galactose. </LI> <LI> <I>E. coli</I> KLPPP used <I>P. palmata</I> hydrolysate efficiently to synthesize succinic acid. </LI> </UL> </P>