BioChem - DOS

Production of cis-11-eicosenoic acid by Mortierella fungi

Journal of applied microbiology

Kikukawa, H.; Sakuradani, E.; Nishibaba, Y.; Okuda, T.; Ando, A.; Shima, J.; Shimizu, S.; Ogawa, J.

<P><B>Abstract</B></P><P><B>Aim</B></P><P>To find <I>cis</I>&#8208;11&#8208;eicosenoic acid (20:1<I>&omega;</I>9, EA)&#8208;producing micro&#8208;organisms.</P><P><B>Methods and Results</B></P><P>We found EA&#8208;producing fungi by screening about 300 fungal strains and identified a major fatty acid accumulated in the <I>Mortierella</I> fungi as EA by means of GC&#8208;MS analysis. In particular, <I>Mortierella chlamydospora </I>CBS 529.75 produced a high amount of EA (36&middot;3？mg？g<SUP>&minus;1</SUP> of dried cells) on cultivation at 28&deg;C for 4？days and then at 12&deg;C for 3？days. In the result of lipid analysis, most of the EA was a component of triacylglycerols, not phospholipids.</P><P><B>Conclusion</B></P><P>We found that <I>M.？chlamydospora </I>CBS 529.75 was the best producer for the microbial production of EA.</P><P><B>Significance and Impact of the Study</B></P><P>EA is beneficial as a raw material for medical supplies and a moisturizing component of cosmetic creams. This is the first report of microbial production of EA.</P>

2015

1364-5072

1365-2672

118

3

pp.641-647

10.1111/jam.12725

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71441183

eicosenoic acid; fatty acid elongase; Mortierella; polyunsaturated fatty acid; screening