초록
In this study, a photoautotrophic hydrogen production of Chlorella pyrenoidosa was presented, with NaHCO<SUB>3</SUB> (10 mM) instead of organic carbon and DCMU addition (10 μM) instead of sulfur-deprivation. The results showed that the algal O<SUB>2</SUB> evolution was significantly inhibited by DCMU, and the earlier it was added the more serious the inhibition was. Overall, when DCMU was added within 9 h after illumination, the relative O<SUB>2</SUB> concentrations could be regarded as anaerobic (<6%). Comparatively, the algal H<SUB>2</SUB> photoproduction was also influenced by DCMU addition time, but with opposite trend to the O<SUB>2</SUB> evolution. In summary, the highest H<SUB>2</SUB> production occurred when DCMU was injected at 9 h, being fourfold of that in sulfur-deprived TAP medium (93.86 mL L<SUP>-1</SUP> vs 23.12 mL L<SUP>-1</SUP>). Further study suggested that the higher H<SUB>2</SUB> productivity might be mainly due to a relatively higher PSII photochemical activity.