Characterization of produced xylanase by Bacillus subtilis D3d newly isolated from apricot phyllosphere and its potential in pre-digestion of BSG
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Characterization of produced xylanase by Bacillus subtilis D3d newly isolated from apricot phyllosphere and its potential in pre-digestion of BSG
학술지
Journal of industrial and engineering chemistry : JIEC
저자명
Moteshafi, H.; Hashemi, M.; Mousavi, S.M.; Mousivand, M.
초록
Bacillus subtilis D3d newly isolated from apricot phyllosphere has the potential of producing high levels of xylanase. The present study used response surface methodology (RSM) to optimize catalytic activity. Factors including temperature, pH and beechwood xylan concentration were optimized using central composite design (CCD). Under optimized condition maximum xylanase activity found 260U/ml. The enzyme showed stability over a range of pH (5.0-9.0) and exhibited more than 80% activity after 72h at 40<SUP>o</SUP>C. Such RSM models as well as stability data can be used at different operational conditions to predict the residual catalytic activity. Regard to pre-digested brewer's spent grain (BSG) has potential as a valuable poultry B. subtilis D3d crude xylanase. Maximum reducing sugar yield were found to be 68.43mg<SUB>reducingsugars</SUB>/g<SUB>BSG</SUB> at optimum condition. The results revealed that B.subtilis D3d is a promising xylanase-producing bacterium with great potential for use in the food and feed industries.
발행연도
2016
발행기관
Korean Society of Industrial and Engineering Chemistry