BioChem - DOS

Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes

Plant biotechnology journal

Tilbrook, Kimberley; Poirier, Yves; Gebbie, Leigh; Schenk, Peer M.; McQualter, Richard B.; Brumbley, Stevens M.

<P><B>Summary</B></P><P>Polyhydroxyalkanoates (PHAs) are bacterial carbon storage polymers used as renewable, biodegradable plastics. PHA production in plants may be a way to reduce industrial PHA production costs. We recently demonstrated a promising level of peroxisomal PHA production in the high biomass crop species sugarcane. However, further production strategies are needed to boost PHA accumulation closer to commercial targets. Through exogenous fatty acid feeding of <I>Arabidopsis thaliana</I> plants that contain peroxisome&#8208;targeted PhaA, PhaB and PhaC enzymes from <I>Cupriavidus necator</I>, we show here that the availability of substrates derived from the &beta;&#8208;oxidation cycle limits peroxisomal polyhydroxybutyrate (PHB) biosynthesis. Knockdown of peroxisomal citrate synthase activity using artificial microRNA increased PHB production levels approximately threefold. This work demonstrates that reduction of peroxisomal citrate synthase activity may be a valid metabolic engineering strategy for increasing PHA production in other plant species.</P>

2014

cc-by

1467-7644

1467-7652

12

8

pp.1044-1052

10.1111/pbi.12211

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70385495

https://eprints.qut.edu.au/114644/1/114644.pdf

polyhydroxyalkanoate; citrate synthase; β&#x2010; oxidation; peroxisome; plant metabolic engineering; Arabidopsis;